Skip to main content

Cartilage matrix glycoprotein is present in serum in experimental canine osteoarthritis.

We have previously described disulfide-bonded 550,000-D cartilage matrix glycoprotein (CMGP), found in the cartilage of normal hyaline, fibrocartilage, and vitreous eye, and is composed of subunits with molecular weights clear of 130,000 at 4% gel (116,000 in 9% gel). In osteoarthritic cartilage of dogs experiencing transection of Porcine Serum the anterior cruciate ligament (ACL), CMGP cleaved fragments major immunoreactive with apparent molecular weight of 65,000 and 75,000 after reduction with 2-mercaptoethanol. 

In this study, using immunolocation analysis, monoclonal antibodies to CMGP not react with serum from 8 of the 12 dogs before ACL transection but reacted with serum from seven of these animals 4 weeks after surgery and the serum of 10 dogs in the sacrifice, 8-14 weeks after ACL transection. Serum from four dogs react with the monoclonal antibody before the ACL transection. Sera from negative two dogs at all time points.

 Immunolocation studies using polyclonal antiserum to CMGP conducted in seven dogs and produced identical results with monoclonal antibodies in four dogs. In contrast, analysis of serial serum samples from three dogs with cartilage atrophy revealed no evidence CMGP at any point of time. These data indicate that CMGP may be a serum marker for osteoarthritis in this canine model
Cartilage matrix glycoprotein is present in serum in experimental canine osteoarthritis.


Clinical studies in dogs hemoglobinuria dirofilarial: measured and calculated serum osmolality and osmolar gap.


serum osmolality and osmolar gap is determined in 43 normal healthy beagles (control group) and 40 dogs with dirofilarial hemoglobinuria (group hemoglobinuria). In the control group, the levels of serum osmolality were measured and calculated in the means 296 +/- 5 (SD) mOsm / kg and 293 +/- 6 mOsm / kg respectively, show the osmolar gap of less than 10 mOsm / kg. In the group hemoglobinuria, measured serum osmolality ranging from 272-370 mOsm / kg. 

A large number of dogs have a normal Porcine Serum Albumin serum osmolality despite severe intravascular haemolysis, indicating that changes in serum osmolality will not be the direct cause of intravascular hemolysis. measured serum osmolality (331 +/- 28 mOsm / kg) were significantly higher in the 11 dogs that died after surgical removal of heartworms than 29 dogs were recovered after deletion (302 +/- 17 mOsm / kg). 

calculated serum osmolality was 296 +/- 16 mOsm / kg in 24 cases recovered, and 304 +/- 22 mOsm / kg in 10 cases are fatal. Osmolar gap stayed in the normal range of 5.4 +/- 5.9 mOsm / kg in the case recover, but achieve a higher level of 22.7 +/- 8.9 mOsm / kg in fatal cases, indicate prognosis worse in cases with large osmolar gap. 

There was a significant positive correlation between osmolality were measured serum and gaps osmolar, serum sodium, potassium, BUN, GOT, GPT, creatinine, bilirubin and hemoglobin values ​​of plasma, as well as between the osmolar gap and serum potassium, BUN, GOT, GPT, creatinine and bilirubin values. Plasma hemoglobin concentration fell sharply without any significant change in serum osmolality 20 hours after the removal of heartworm.
Labels:

Comments

Post a Comment

Popular posts from this blog

Spontaneous benign prostatic hyperplasia in the beagle. Age-associated changes in serum hormone levels, and the morphology and secretory function of the canine prostate.

This paper is a cross-sectional study of spontaneous benign prostatic hyperplasia (BPH) in a single canine species. The effects of aging and hormonal changes in growth, histology and function of the prostate gland secretion of dogs studied in 42 male beagle dogs aged between 8 mo to 9 yrs. Beagle enlarged prostate for at least 6 years, whether normal or hyperplastic. In contrast, prostate secretory function , determined  Rabbit Serum by the volume of ejaculate and total protein ejaculation, decreased sharply after 4 years of age. growth and functional changes in the prostate reciprocal closely associated with a progressive increase in the incidence of BPH, which is already evident in some dogs at the age of two. By the age of simple decline in serum androgen levels with no apparent change in serum 17 beta-estradiol levels.  This indicates that the growth and functional changes associated with the development of BPH and begins very early in life change reflects the sensitivity...
Post a Comment
Read more

Rate of binding of antibiotics to canine serum protein.

The current level of the three antibiotics bind similar chemical structure, each with a different degree of protein binding, is determined. Cephaloridine, which is 10% bound to serum proteins, tied at a faster rate than cephalothin, which is 40% bound to  Rat Plasma serum proteins. Cefazolin, bound 80%, is required for a maximum period of maximum binding occurred.  Binding protein level appear directly related to the total percentage bound. Data from these studies indicate that prolonged levels of the drug binds highly protein-bound may affect pharmacological studies. Canine marrow transplantation: the serum blocking factors necessary to maintain stable chimeric state? Long-term radiation chimera nine dogs and they dog ​​histocompatibility locus (DL-A) compatible, negative cultural mix littermate donor leukocytes marrow studied between 545 and 1282 days after total body irradiation 1,200 R and marrow grafting. Before the time of testing, the donor marrow chimeras are immunize...
Post a Comment
Read more