We have previously described disulfide-bonded 550,000-D cartilage matrix glycoprotein (CMGP), found in the cartilage of normal hyaline, fibrocartilage, and vitreous eye, and is composed of subunits with molecular weights clear of 130,000 at 4% gel (116,000 in 9% gel). In osteoarthritic cartilage of dogs experiencing transection of Porcine Serum the anterior cruciate ligament (ACL), CMGP cleaved fragments major immunoreactive with apparent molecular weight of 65,000 and 75,000 after reduction with 2-mercaptoethanol.
In this study, using immunolocation analysis, monoclonal antibodies to CMGP not react with serum from 8 of the 12 dogs before ACL transection but reacted with serum from seven of these animals 4 weeks after surgery and the serum of 10 dogs in the sacrifice, 8-14 weeks after ACL transection. Serum from four dogs react with the monoclonal antibody before the ACL transection. Sera from negative two dogs at all time points.
Immunolocation studies using polyclonal antiserum to CMGP conducted in seven dogs and produced identical results with monoclonal antibodies in four dogs. In contrast, analysis of serial serum samples from three dogs with cartilage atrophy revealed no evidence CMGP at any point of time. These data indicate that CMGP may be a serum marker for osteoarthritis in this canine model
Clinical studies in dogs hemoglobinuria dirofilarial: measured and calculated serum osmolality and osmolar gap.
serum osmolality and osmolar gap is determined in 43 normal healthy beagles (control group) and 40 dogs with dirofilarial hemoglobinuria (group hemoglobinuria). In the control group, the levels of serum osmolality were measured and calculated in the means 296 +/- 5 (SD) mOsm / kg and 293 +/- 6 mOsm / kg respectively, show the osmolar gap of less than 10 mOsm / kg. In the group hemoglobinuria, measured serum osmolality ranging from 272-370 mOsm / kg.
A large number of dogs have a normal Porcine Serum Albumin serum osmolality despite severe intravascular haemolysis, indicating that changes in serum osmolality will not be the direct cause of intravascular hemolysis. measured serum osmolality (331 +/- 28 mOsm / kg) were significantly higher in the 11 dogs that died after surgical removal of heartworms than 29 dogs were recovered after deletion (302 +/- 17 mOsm / kg).
calculated serum osmolality was 296 +/- 16 mOsm / kg in 24 cases recovered, and 304 +/- 22 mOsm / kg in 10 cases are fatal. Osmolar gap stayed in the normal range of 5.4 +/- 5.9 mOsm / kg in the case recover, but achieve a higher level of 22.7 +/- 8.9 mOsm / kg in fatal cases, indicate prognosis worse in cases with large osmolar gap.
There was a significant positive correlation between osmolality were measured serum and gaps osmolar, serum sodium, potassium, BUN, GOT, GPT, creatinine, bilirubin and hemoglobin values of plasma, as well as between the osmolar gap and serum potassium, BUN, GOT, GPT, creatinine and bilirubin values. Plasma hemoglobin concentration fell sharply without any significant change in serum osmolality 20 hours after the removal of heartworm.
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