Sera puppies weaned from, specific pathogen-derived hysterectomy free (SPF) closed dog colony does not contain antibodies against TGE virus related or serologically related, but the sera of puppies and dogs that are older than the open cage did.
Antivirus higher titer in the Rat Serum Albumin serum of older dogs suggest that these animals have suffered from either persistent or recurrent infection. Seventy-two puppies have had no contact with pigs, suggesting that the virus can spread independently of any contact with pigs.
Tumor-blocking and -inhibitory serum factor in the clinical course canine venereal tumor.
An in vitro method for growing colonies of cells in a tumor dog sex transplantabel semisolid agar described. Using autologous or homologous sera collected a normal dog as a feeder layer, 49.3 +/- 3.5 and 47.5 +/- 4.5 colonies of tumor is obtained, respectively, when 2 X 10 cells (4) tumors plated. With this test system, the assessment of the number of colonies provide fast and accurate technique for monitoring the serum factor in tumor-bearing dogs https://gentaur.at/ that inhibits colony formation, or blocked inhibition of colony formation.
In 52 dogs given transplants of tumors, demonstrated a direct correlation between the activity of the serum was tested in vitro and in vivo tumor growth characteristics. tumor cells preincubated with the serum of dogs with active tumor growth consistently showed normal colony growth when cultured in agar containing sera colony-inhibitory (blocking effect).
In vivo regression characterized by loss of blocking serum and the development of colony-inhibitory activity of serum in the culture. In metastatic disease, only the blocking activity can be identified, while the persistent locally invasive disease characterized by low levels of both blocking and serum inhibitory activity. The sensitivity of this technique in vivo coupled with predictive capabilities provide a model to monitor serological responses to natural neoplasm in a large, randomly bred animals.
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