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Transmissible gastroenteritis (TGE) of swine: canine serum antibodies against an associated virus.

Sera puppies weaned from, specific pathogen-derived hysterectomy free (SPF) closed dog colony does not contain antibodies against TGE virus related or serologically related, but the sera of puppies and dogs that are older than the open cage did.  A ntivirus higher titer in the Rat Serum Albumin serum of older dogs suggest that these animals have suffered from either persistent or recurrent infection. Seventy-two puppies have had no contact with pigs, suggesting that the virus can spread independently of any contact with pigs. Tumor-blocking and -inhibitory serum factor in the clinical course canine venereal tumor. An in vitro method for growing colonies of cells in a tumor dog sex transplantabel semisolid agar described. Using autologous or homologous sera collected a normal dog as a feeder layer, 49.3 +/- 3.5 and 47.5 +/- 4.5 colonies of tumor is obtained, respectively, when 2 X 10 cells (4) tumors plated. With this test system, the assessment of the number of colonies provide fa...
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Rate of binding of antibiotics to canine serum protein.

The current level of the three antibiotics bind similar chemical structure, each with a different degree of protein binding, is determined. Cephaloridine, which is 10% bound to serum proteins, tied at a faster rate than cephalothin, which is 40% bound to  Rat Plasma serum proteins. Cefazolin, bound 80%, is required for a maximum period of maximum binding occurred.  Binding protein level appear directly related to the total percentage bound. Data from these studies indicate that prolonged levels of the drug binds highly protein-bound may affect pharmacological studies. Canine marrow transplantation: the serum blocking factors necessary to maintain stable chimeric state? Long-term radiation chimera nine dogs and they dog ​​histocompatibility locus (DL-A) compatible, negative cultural mix littermate donor leukocytes marrow studied between 545 and 1282 days after total body irradiation 1,200 R and marrow grafting. Before the time of testing, the donor marrow chimeras are immunize...

Spontaneous benign prostatic hyperplasia in the beagle. Age-associated changes in serum hormone levels, and the morphology and secretory function of the canine prostate.

This paper is a cross-sectional study of spontaneous benign prostatic hyperplasia (BPH) in a single canine species. The effects of aging and hormonal changes in growth, histology and function of the prostate gland secretion of dogs studied in 42 male beagle dogs aged between 8 mo to 9 yrs. Beagle enlarged prostate for at least 6 years, whether normal or hyperplastic. In contrast, prostate secretory function , determined  Rabbit Serum by the volume of ejaculate and total protein ejaculation, decreased sharply after 4 years of age. growth and functional changes in the prostate reciprocal closely associated with a progressive increase in the incidence of BPH, which is already evident in some dogs at the age of two. By the age of simple decline in serum androgen levels with no apparent change in serum 17 beta-estradiol levels.  This indicates that the growth and functional changes associated with the development of BPH and begins very early in life change reflects the sensitivity...

Sensitivity and specificity of canine serum total amylase and isoamylase activity determinations.

isoamylases serum prospectively determined in dogs with pancreatic disease and extrapancreatic. Determination of serum isoamylase means significantly different (p less than 0.05) between normal dogs and dogs with pancreatitis and  Rabbit Antiserum exocrine pancreatic insufficiency. The sensitivity of the determination of serum amylase activity isoamylase exceeded the total for the diagnosis of pancreatitis. determination of serum isoamylase less affected by the disease extrapancreatic than total amylase activity when used in the diagnosis of pancreatic diseases.  Both serum isoamylase or the determination of total amylase activity has a sensitivity sufficient to support its use in the diagnosis of exocrine pancreatic insufficiency. There was a significant (p less than 0.05) linear correlation between the determination of isoamylase, total amylase activity, and trypsin-like immunoreactivity concentration. serum antibody response to canine parvovirus, canine adenovirus-1, and ...

Cartilage matrix glycoprotein is present in serum in experimental canine osteoarthritis.

We have previously described disulfide-bonded 550,000-D cartilage matrix glycoprotein (CMGP), found in the cartilage of normal hyaline, fibrocartilage, and vitreous eye, and is composed of subunits with molecular weights clear of 130,000 at 4% gel (116,000 in 9% gel). In osteoarthritic cartilage of dogs experiencing transection of  Porcine Serum the anterior cruciate ligament (ACL), CMGP cleaved fragments major immunoreactive with apparent molecular weight of 65,000 and 75,000 after reduction with 2-mercaptoethanol.  In this study, using immunolocation analysis, monoclonal antibodies to CMGP not react with serum from 8 of the 12 dogs before ACL transection but reacted with serum from seven of these animals 4 weeks after surgery and the serum of 10 dogs in the sacrifice, 8-14 weeks after ACL transection. Serum from four dogs react with the monoclonal antibody before the ACL transection. Sera from negative two dogs at all time points.  Immunolocation studies using polyclona...

Measurement of Neutrophil Gelatinase-Associated Lipocalin Concentration in Canine Cerebrospinal Fluid and Serum and Its Involvement in Neuroinflammation.

Neutrophil gelatinase-associated lipocalin (NGAL) is a glycoprotein is involved in inflammation acts as an acute phase protein and chemokines as well as a regulator of iron homeostasis. NGAL has proved to be upregulated in experimental autoimmune encephalomyelitis (EAE) in mice. Increasing concentrations of NGAL in  Ovine Serum the cerebrospinal fluid (CSF) and the expression in the central nervous system (CNS) have been described in human neuroinflammatory diseases such as multiple sclerosis and lupus, neuropsychiatric and bacterial meningitis.  We aimed to investigate the involvement of NGAL in spontaneous nerve inflammation dog as an animal model of the potential immune-mediated neurological disorders. A commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of NGAL dog CSF validated for use in dogs. Concentration in CSF and serum of patients suffering from steroid-responsive dog meningitis- arteriitis (SRMA), Meningoencephalitis of unknown...